Pair Name	Tenacissoside G, Fluorouracil
Disease Info	[ICD-11: 2B91]	Colorectal cancer		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Up-regulation	Cleavage	CASP3	hsa836
	Up-regulation	Cleavage	CASP8	hsa841
	Up-regulation	Cleavage	CASP9	hsa842
	Down-regulation	Expression	CCND1	hsa595
	Down-regulation	Expression	CDK2	hsa1017
	Down-regulation	Expression	CDK4	hsa1019
	Down-regulation	Expression	CDK6	hsa1021
	Up-regulation	Expression	CDKN1A	hsa1026
	Up-regulation	Phosphorylation	TP53	hsa7157
In Vitro Model	RKO	Colon carcinoma	Homo sapiens (Human)	CVCL_0504
In Vivo Model	RKO cells at a density of 3×10⁶/ml were suspended in cold PBS, and a volume of 100 μl cell suspension was administrated subcutaneously in the right flank of each mouse.
Result	TG potentiated 5-FU's inhibitory activity to human colorectal cancer through arresting cell cycle progression and inducing p53-mediated apoptosis, which may present a novel strategy in CRC therapies and contribute to the optimizing clinical application of 5-FU.